Recombinant Human IL2RA protein(Met1-Cys213), hFc-tagged

• Cat.No.: IL2RA-636H
• Product Overview: Recombinant Human IL2RA (NP_000408.1)(Met 1-Cys 213) was expressed in HEK293 with C-terminal fused Human IgG1 Fc region.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 1-213 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Recombinant Human IL2 Protein, Low Endotoxin at 2 μg/ml (100 μl/well) can bind CD25/IL2RA Protein, Human, Recombinant (ECD, hFc Tag), The EC50 is 3-10 ng/mL.
• Molecular Mass: The mature recombinant human IL2Rα/Fc is a disulfide-linked homodimeric protein generated by proteolytic removal of the signal peptide. The reduced monomer consists of 430 amino acids and has a predicted molecular mass of 48.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhIL2Rα/Fc monomer is approximately 72 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL2RA interleukin 2 receptor, alpha [ Homo sapiens ]
• Official Symbol: IL2RA
• Synonyms: IL2RA; interleukin 2 receptor, alpha; IL2R; interleukin-2 receptor subunit alpha; CD25; p55; IL2-RA; IL-2-RA; TAC antigen; IL-2R subunit alpha; IL-2 receptor subunit alpha; TCGFR; IDDM10
• Gene ID: 3559
• mRNA Refseq: NM_000417
• Protein Refseq: NP_000408
• MIM: 147730
• UniProt ID: P01589