Recombinant Human IL3, His-tagged
Cat.No. : | IL3-56H |
Product Overview : | Recombinant Human Interleukin-3 produced inE.Coliis single, a non-glycosylated, Polypeptide chain containing 133 amino acids fragment (20-152) and having a total molecular mass of 15 kDa with an amino-terminal hexahistidine tag. The IL3 His is purified by proprietary chromatographic techniques. |
- Specification
- Gene Information
- Related Products
Cat. No. : | IL3-56H |
Description : | Interleukin-3 is a pleiotropic cytokine produced primarily by activated T cells. IL-3 is thought to function via specific cell surface receptors to stimulate the proliferation, differentiation and survival of haematopoietic cell lines. IL-3 has also been shown to affect the functional activity of a variety of other cell types including mast cells, eosinophils, megakaryocytes and basophils. |
Source : | Escherichia Coli. |
Physical Appearance : | Sterile Filtered clear solution. |
Purity : | Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Formulation : | Interleukin-3 His is supplied in 1x PBS and 50% glycerol. |
Biological activity : | The ED50 for this effect is<0.53 ng/ml . measured in a cell proliferation assayusing tf1 human erythroleukemic>0.53> |
Activity Assay : | 1.Cell line: TF1 (human erythroleukemic cell)2.Maintenance Condition: 10% FBS RPMI 1640 with GM-CSF3.Assay Medium: 10% FBS RPMI 1640 without GM-CSF4.Cell Density: 2 x 104 cells/well (96 well plate, final volume 100ul)5.Starvation : 24hr, Assay medium6.Incubation Time : 48 hr (after sample treatment)7.Concentration Range: 0.048ng/ml - 25ng/ml 8.Detection method : MTT assay |
Storage : | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles. |
Gene Name : | IL3 interleukin 3 (colony-stimulating factor, multiple) [ Homo sapiens ] |
Synonyms : | IL3; interleukin 3 (colony-stimulating factor, multiple); IL-3; MCGF; MGC79398; MGC79399; MULTI-CSF; interleukin 3; mast-cell growth factor; P-cell stimulating factor; hematopoietic growth factor; Mast cell growth factor; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; Hematopoietic growth factor |
Gene ID : | 3562 |
mRNA Refseq : | NM_000588 |
Protein Refseq : | NP_000579 |
MIM : | 147740 |
UniProt ID : | P08700 |
Chromosome Location : | 5q31.1 |
Pathway : | Cytokine-cytokine receptor interaction; Fc epsilon RI signaling pathway; Hematopoietic cell lineage; Jak-STAT signaling pathway; Apoptosis; Asthma |
Function : | cytokine activity; growth factor activity; protein binding; interleukin-3 receptor binding |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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