Active Recombinant Human IL6 protein, His-tagged

Name: Active Recombinant Human IL6 protein, His-tagged
SKU: IL6-002H

Species :	Human
Source :	E.coli
Tag :	His
Protein Length :	29-212 aa
Form :	Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Bio-activity :	Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. Interleukin 6 is secreted by T cells and macrophages to stimulate immune response and also plays a role in fighting infection. It supports the growth of B cells and is antagonistic to regulatory T cells. To test the effect of IL6 on cell proliferation, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 20,000 cells/well with 10% serum standard 1640 which contains various concentrations of recombinant human IL6. After incubated for 3 days, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of TF-1 cells after incubation with rhIL6 for 3 days observed by inverted microscope was shown. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant human IL6 for 3 days. It was obvious that recombinant human IL6 significantly increased cell viability of TF-1 cells, the EC50 was 0.016 ug/ml.
Molecular Mass :	24.7kDa
Endotoxin :	<1.0EU per 1μg (determined by the LAL method).
Purity :	> 90% as determined by SDS-PAGE.
Storage :	Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
Reconstitution :	Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Name	IL6 interleukin 6 (interferon, beta 2) [ Homo sapiens ]
Official Symbol	IL6
Synonyms	IL6; interleukin 6 (interferon, beta 2); IFNB2; interleukin-6; BSF2; HGF; HSF; IL 6; CDF; BSF-2; IFN-beta-2; interferon beta-2; interleukin BSF-2; hybridoma growth factor; CTL differentiation factor; B-cell stimulatory factor 2; B-cell differentiation factor; IL-6;
Gene ID	3569
mRNA Refseq	NM_000600
Protein Refseq	NP_000591
MIM	147620
UniProt ID	P05231