Recombinant Human ILK Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: ILK-5726H
• Product Overview: ILK MS Standard C13 and N15-labeled recombinant protein (NP_001014794) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 51.4 kDa
• AA Sequence: MDDIFTQCREGNAVAVRLWLDNTEN DLNQGDDHGFSPLHWACREGRSAVV EMLIMRGARINVMNRGDDTPLHLAA SHGHRDIVQKLLQYKADINAVNEHG NVPLHYACFWGQDQVAEDLVANGAL VSICNKYGEMPVDKAKAPLRELLRE RAEKMGQNLNRIPYKDTFWKGTTRT RPRNGTLNKHSGIDFKQLNFLTKLN ENHSGELWKGRWQGNDIVVKVLKVR DWSTRKSRDFNEECPRLRIFSHPNV LPVLGACQSPPAPHPTLITHWMPYG SLYNVLHEGTNFVVDQSQAVKFALD MARGMAFLHTLEPLIPRHALNSRSV MIDEDMTARISMADVKFSFQCPGRM YAPAWVAPEALQKKPEDTNRRSADM WSFAVLLWELVTREVPFADLSNMEI GMKVALEGLRPTIPPGISPHVCKLM KICMNEDPAKRPKFDMIVPILEKMQ DKTRTRPLEQKLISEEDLAANDILD YKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ILK integrin-linked kinase [ Homo sapiens (human) ]
• Official Symbol: ILK
• Synonyms: ILK; integrin-linked kinase; integrin-linked protein kinase; ILK-1; p59ILK; integrin-linked kinase-2; 59 kDa serine/threonine-protein kinase; P59; ILK-2; DKFZp686F1765
• Gene ID: 3611
• mRNA Refseq: NM_001014794
• Protein Refseq: NP_001014794
• MIM: 602366
• UniProt ID: Q13418