Recombinant Human INSL3 Protein, His-tagged

• Cat.No.: INSL3-722H
• Product Overview: Recombinant Human INSL3 fused with His tag at C-terminal was expressed in HEK293 cells.

Specification

• Description: This gene encodes a member of the insulin-like hormone superfamily. The encoded protein is mainly produced in gonadal tissues. Studies of the mouse counterpart suggest that this gene may be involved in the development of urogenital tract and female fertility. This protein may also act as a hormone to regulate growth and differentiation of gubernaculum, and thus mediating intra-abdominal testicular descent. Mutations in this gene may lead to cryptorchidism. Alternate splicing results in multiple transcript variants.
• Source: HEK293 cells
• Species: Human
• Tag: His
• Form: Lyophilized from a 0.2 µM filtered solution of 20mM PB, 150mM NaCl, pH7.4
• Molecular Mass: 13.4kD
• AA Sequence: LGPAPTPEMREKLCGHHFVRALVRV CGGPRWSTEARRPATGGDRELLQWL ERRHLLHGLVADSNLTLGPGLQPLP QTSHHHRHHRAAATNPARYCCLSGC TQQDLLTLCPYVDHHHHHH
• Endotoxin: Endotoxin level is <0.1 ng/µg of protein (<1EU/µg).
• Purity: >95% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Gene Information

• Gene Name: INSL3 insulin-like 3 (Leydig cell) [ Homo sapiens ]
• Official Symbol: INSL3
• Synonyms: INSL3; insulin-like 3 (Leydig cell); relaxin like factor , RLNL; insulin-like 3; MGC119818; MGC119819; RLF; ley-I-L; relaxin-like factor b; leydig insulin-like peptide; leydig insulin -like hormone; leydig insulin -like peptide; RLNL
• Gene ID: 3640
• mRNA Refseq: NM_005543
• Protein Refseq: NP_005534
• MIM: 146738
• UniProt ID: P51460

Reviews

03/26/2019

User-friendly data analysis capabilities contributed to the product's overall value for our research.

11/28/2018

The product's adaptability to custom protocols empowered us to explore innovative experimental setups.

07/19/2017

The ability to use the product across different scientific disciplines broadened the scope of our investigations.

Q&As

In which tissues is the expression of INSL3 prominent?

INSL3 is prominently expressed in the testes, but it is also found in the ovaries and other tissues.

Are there any developmental stages where INSL3 expression is crucial?

During fetal development, INSL3 expression is crucial for proper testicular descent, a process essential for male reproductive health.

How is INSL3 regulated at the transcriptional level?

INSL3 is regulated at the transcriptional level by various factors, including hormones such as luteinizing hormone (LH).

How does INSL3 contribute to reproductive physiology?

INSL3 plays a vital role in reproductive physiology by promoting testicular descent during fetal development and supporting spermatogenesis in adulthood.

Does INSL3 have functions beyond reproduction?

Beyond reproduction, INSL3 may have metabolic effects, potentially influencing bone metabolism and insulin sensitivity.

What is the role of INSL3 in the female reproductive system?

In the female reproductive system, INSL3 is involved in ovarian follicle development and is produced by theca cells.

Is there a correlation between INSL3 levels and fertility in males?

Research suggests a correlation between INSL3 levels in semen and male fertility, making it a potential biomarker.