Recombinant Human Insulin-like Growth Factor 2 (somatomedin A)

• Cat.No.: IGF2-609H
• Product Overview: Recombinant humanIGF2 protein was expressed in E. coli. They are made by reacting hIGF-II withSulfo-NHS-Biotin reagent. In di-biotinyl-human-IGF-II both the a-amino groupof the N-terminal alanine and the e-amino group of the lysine at position 65are biotinylated.

Specification

• Cat. No.: IGF2-609H
• Description: IGF2 is one ofthree protein hormones that share structural similarity to insulin. IGF2 iscreated by Theca cells to act in an autocrine manner on the theca cellsthemselves, and in a paracrine manner on Granulosa cells in the ovary. IGF2promotes granulosa cell proliferation during the follicular phase of themenstrual cycle, acting alongside Follicle Stimulating Hormone.
• Source: Following thereaction of hIGF-II with Sulfo-NHS-Biotin reagent , the mono- anddi-biotinyl-human-IGF-II are separated from the other reaction products byHPLC. The mono-biotinyl-human-IGF-II and the di-biotinyl-human-IGF-II peaksare then further purified by HPLC to yield Receptor Grade products.
• Species: Human
• Purity: Greater than 95 %(by HPLC)
• Molecular Weight: Mono-biotinyl-human-IGF-II-7696daltons; Di-biotinyl-human-IGF-II-7922 daltons; Homogeneity and mass of bothconfirmed by Mass Spectrometry.
• N-terminal sequence analysis: 5 residues Greaterthan 95 % single sequence.
• Biological Activity: Western ligand blotto bovine IGFBP-2.
• Endotoxin: Less than 0.1 EU/μg
• State and appearance: Lyophilized whitepowder. Dried from 0.1 M acetonitrile/ 0.1% trifluoroacetic acid and storedunder dry nitrogen at a slight vacuum (-25 kPa).
• Reconstitution: Handling of GroPepIGF-I, IGF-II and IGF Analogs.
• Storage: At least 2 years at2 - 4°C (lyophilized).
• OfficialSymbol: IGF2
• Pathways: Apoptosis; Diabetespathways; Endochondral Ossification; Hedgehog Signaling Pathway; Regulationof Insulin-like Growth Factor (IGF) Activity by Insulin-like Growth FactorBinding Proteins (IGFBPs)

Gene Information

• Gene Name: IGF2 insulin-like growth factor2 (somatomedin A) [ Homo sapiens ]
• Synonyms: IGF2;insulin-like growth factor 2 (somatomedin A); IGF-II; PP9974; C11orf43;FLJ22066; FLJ44734; insulin-like growth factor II;somatomedin-A; OTTHUMP00000011012; OTTHUMP00000011013; OTTHUMP00000011015;OTTHUMP00000011018; OTTHUMP00000011157; OTTHUMP00000011158;OTTHUMP00000220677; insulin-like growth factor type 2; chromosome11 open reading frame 43; Insulin-like growth factor II; Somatomedin-A
• Gene ID: 3481
• mRNA Refseq: NM_001127598
• Protein Refseq: NP_001121070
• MIM: 147470
• UniProt ID: P01344
• Chromosome Location: 11p15.5
• Function: growth factoractivity; hormone activity; insulin receptor binding; insulin-like growthfactor receptor binding; protein binding; protein serine/threonine kinaseactivator activity; receptor activator activity