Recombinant Human Insulin-like Growth Factor 2 (Somatomedin A)

• Cat.No.: IGF2-450H
• Product Overview: Recombinant Human Insulin-like Growth Factor 2 (Somatomedin A) is expressed inE.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Human Des(1-6) Insulin-like Growth Factor-II (Des(1-6)IGF-II) is a 61 amino acid analog of human IGF-II, lacking the first six N-terminal amino acids. This analog of IGF-II binds to the Type 1 and Type 2 IGF receptors with similar affinity to IGF-II. Human Des(1-6)IGF-II exhibits reduced affinity for IGF binding proteins (IGFBPs), which modulate the actions of IGFs.
• Molecular Weight: 6765 daltons – confirmed by Mass Spectrometry.
• N-terminal Sequence Analysis: 5 residues > 95 % single sequence.
• Purity: > 95 % (by HPLC and N-terminal sequence analysis).
• Biological Activity: Type 1 IGF receptor binding assay: ED50 < 50 ng/ml; Type 2 IGF receptor binding assay: ED50 < 100 ng/ml;IGF binding protein assay: ED50 > 1000 ng/ml; Stimulation of protein synthesis in rat L6 myoblasts: ED50 < 75 ng/ml.
• Endotoxin: < 0.1 EU/µg.
• State And Appearance: Lyophilized white powder. Dried from 0.1 M acetic acid and stored under nitrogen at a slight vacuum.
• Detection: Determination of IGF-I or IGF-II in a Range of Species by Radioimmunoassay (RIA).
• Reconstitution: Handling of Novozymes GroPep IGF-I, IGF-II and IGF Analogs.
• Storage/Stability: At least 2 years at 2 - 4°C (lyophilized).

Gene Information

• Gene Name: IGF2 insulin-like growth factor 2 (somatomedin A) [ Homo sapiens ]
• Synonyms: insulin-like growth factor 2 (somatomedin A);INSIGF; pp9974; C11orf43; FLJ22066; FLJ44734; IGF2; insulin-like growth factor 2; IGF-II; somatomedin A; somatomedin-A; insulin-like growth factor II; insulin-like growth factor type 2; putative insulin-like growth factor II associated protein; chromosome 11 open reading frame 43; OTTHUMP00000011157; OTTHUMP00000011018
• Gene ID: 3481
• mRNA Refseq: NM_000612
• Protein Refseq: NP_000603
• MIM: 147470
• UniProt ID: P01344
• Chromosome Location: 11p15.5
• Pathway: Diabetes pathways
• Function: growth factor activity; hormone activity; insulin receptor binding; insulin-like growth factor receptor binding; protein binding