Recombinant Human Interferon, Alpha 2, His-tagged

• Cat.No.: IFNA2-17H
• Product Overview: Recombinant Human Interferon Alpha 2a produced in Plant is a single, glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19 kDa. The Interferon-2a contains affinity 6xHis tag on C-terminus. The IFN-A2a is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: Nicotiana Sp.
• Tag: His
• Description: IFN-alpha is produced by macrophages and has antiviral activities. Interferon stimulates the production of two enzymes: protein kinase and an oligoadenylate synthetase.
• Amino Acid Sequence: The sequence of the first twelve N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln-Thr-His-Ser-Leu-Gly-Ser-Arg.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 99.0% as determined by both: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: Lyophilized, containing 50% SDS.
• Solubility: It is recommended to reconstitute the lyophilized Interferon-alpha 2a in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The specific activity was determined by a quantitative gene report bioassay using human Type I interferon-sensitive cells. Compared with bacterial derived recombinant IFN-a 2a, assayed in the same conditions, plant derived IFN-alpha 2a showed 6.1 × 10⁸Units/mg in reference to a viral resistance assay using bovine kidney MDBK cells.
• Storage: Lyophilized Interferon alpha 2a although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution IFN-alpha 2a should be stored at 4℃ between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: IFNA2 interferon, alpha 2 [ Homo sapiens ]
• Synonyms: IFNA2; Interferon, Alpha 2; IFNA; INFA2; MGC125764; MGC125765; Interferon alpha A; Alpha-2a interferon; Interferon alpha 2b; OTTHUMP00000021143; Interferon alpha-A; LeIF A; Interferon, alpha-2
• Gene ID: 3440
• mRNA Refseq: NM_000605
• Protein Refseq: NP_000596
• MIM: 147562
• UniProt ID: P01563
• Chromosome Location: 9p22
• Pathway: Autoimmune thyroid disease; Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Natural killer cell mediated cytotoxicity; RIG-I-like receptor signaling pathway; Regulation of autophagy; Toll-like receptor signaling pathway; Antigen processing and presentation
• Function: cytokine activity; interferon-alpha/beta receptor binding