Recombinant Human Interleukin 18 Receptor Accessory Protein, Fc-tagged

Cat.No. : IL18RAP-1658H
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Cat. No. : IL18RAP-1658H
Description : Beta chain of interleukin-18 receptor (IL-18Rbeta) is an accessory protein-like molecule that is required for signaling IL-18 signals through its specific cell surface receptor IL-18R, which comprises two subunits: IL-18Rα and IL-18Rβ. IL-18Rα alone has a weak affinity for IL-18 binding, while the IL-18Rα/β complex has a high affinity. IL-18 Rβ is detected in numerous tissues including lung, spleen, leukocytes and colon.
Molecular Weight : The predicted molecular weight of Recombinant Human IL-18 Rβ is Mr 65.1 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 90-100 kDa.
Source : Mammalian cells
State Of Matter : Lyophilized.
Purity : >90% by SDS Page and analyzed by silver stain.
Endotoxin : <1.0 EU/µg as determined by the LAL method.
Biological Activity : The biological activity of Human IL-18 Rβ was determined byits ability to inhibit IL-18 induced response on KG-1 cells, in the presence of soluble IL-18 Rα/Fc. The expected ED50 for this effect is typically 2 - 6 μg/mL in the presence of 30 μg/mL of soluble IL-18 Rα/Fc and 40 ng/mL of human IL-18.
Storage And Stability : This lyophilized protein is stable for six to twelve months when stored desiccated at -20℃ to -70℃. After aseptic reconstitution, this protein may be stored at 2℃ to 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Gene Name : IL18RAP interleukin 18 receptor accessory protein [ Homo sapiens ]
Synonyms : IL18RAP; interleukin 18 receptor accessory protein; ACPL; CD218b; IL18RB; CDw218b; MGC120589; MGC120590; IL-18 receptor beta; accessory protein-like; AcPL; IL-18RAcP; IL-18Rbeta; IL-1R7; IL-1RAcPL; OTTHUMP00000161337; CD218 antigen-like family member B; CD218b antigen; IL-18 receptor accessory protein; IL-1R accessory protein-like
Gene ID : 8807
mRNA Refseq : NM_003853
Protein Refseq : NP_003844
MIM : 604509
UniProt ID : O95256
Chromosome Location : 2q12
Pathway : Cytokine-cytokine receptor interaction
Function : transmembrane receptor activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What experimental methods are commonly employed to study the expression patterns and regulation of IL18RAP across different cell types and conditions? 12/01/2021

RT-qPCR, immunohistochemistry, and single-cell RNA sequencing characterize IL18RAP expression levels and regulation in diverse cellular contexts.

What techniques are utilized to investigate the role of IL18RAP in immune responses, inflammation, and diseases, and how do researchers assess its potential as a therapeutic target? 03/15/2021

Animal models, patient samples, and therapeutic interventions elucidate IL18RAP's involvement in immune-related disorders and its potential as a therapeutic target.

Could you provide insights into the experimental approaches used to explore the functional effects of IL18RAP isoforms and their specific contributions to IL18 signaling? 12/21/2020

Isoform-specific knockdown, mutagenesis, and functional assays delineate IL18RAP isoforms' distinct roles in modulating IL18-mediated responses.

How are co-immunoprecipitation, fluorescence resonance energy transfer (FRET), and biophysical assays applied to characterize the interactions between IL18RAP, IL18, and IL18R1? 12/20/2019

Co-immunoprecipitation assays, FRET studies, and biophysical techniques characterize IL18RAP's interactions with IL18 and IL18R1 partners.

What experimental methods are used to elucidate the effects of post-translational modifications, such as glycosylation, on IL18RAP's structure and function? 05/18/2019

Mass spectrometry, mutagenesis, and functional assays investigate the impact of post-translational modifications on IL18RAP's conformation and function.

How do researchers experimentally determine the binding affinity and kinetics of IL18RAP to IL18 and IL18R1, considering its pivotal role in forming the IL18 receptor complex? 11/17/2018

Surface plasmon resonance, bio-layer interferometry, and surface plasmon-enhanced fluorescence assays quantify IL18RAP's binding kinetics to IL18 and IL18R1.

Can you explain the use of knockdown, knockout, and gene editing techniques to uncover the physiological importance of IL18RAP in mediating IL18 signaling? 08/10/2018

siRNA knockdown, CRISPR/Cas9-mediated knockout, and transgenic models unravel IL18RAP's physiological roles and contributions to IL18 signaling.

How are advanced imaging techniques, such as single-molecule imaging and super-resolution microscopy, utilized to visualize the dynamic assembly of the IL18 receptor complex involving IL18RAP? 11/05/2017

Single-molecule imaging, super-resolution microscopy, and live-cell imaging visualize the dynamic assembly of IL18 receptor complexes involving IL18RAP.

Can you elaborate on the strategies used to investigate the structural features of IL18RAP's interaction interfaces with IL18 and IL18R1, and how these interfaces contribute to receptor activation? 10/29/2017

X-ray crystallography, NMR spectroscopy, and computational modeling reveal IL18RAP's structural interactions with IL18 and IL18R1 domains.

How do researchers dissect the intracellular signaling pathways triggered by IL18RAP upon binding to IL18 and its collaboration with IL18R1? 10/09/2017

Phosphoprotein analysis, pathway-specific inhibitors, and functional assays dissect IL18RAP's intracellular signaling cascades and its crosstalk with IL18R1.

Customer Reviews (3)

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Reviews
02/09/2021

    Effective in signaling studies.

    11/17/2020

      High specificity.

      09/16/2018

        Reliable in co-immunoprecipitation.

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