| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
Flag&His |
| Protein Length : |
34-179aa |
| Description : |
Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT‑1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family. |
| Tag : |
C-His&Flag |
| Molecular Mass : |
19.4 kDa |
| Endotoxin : |
Less than 1.0 EU/μg by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Storage : |
Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Storage Buffer : |
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol. |
| Reconstitution : |
Reconstitute at 250 μg/mL in sterile water. |
