Recombinant Human Interleukin 9, HQ-tagged IL9-5342H

Recombinant Human Interleukin 9, HQ-tagged


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Cat.No. : IL9-5342H Optional Service: Optional requirements on this protein
Product Overview : Recombinant humanIL9 contains 127 amino acids and a 16 aa HQ tag. Total length is 143 aa.
Description : Interleukin 9, alsoknown as IL9, is a cytokine (cell signalling molecule) belonging to the groupof interleukins. The protein encoded by this gene is a cytokine produced byT-cells and specifically by CD4+ helper cells that acts as a regulator of avariety of hematopoietic cells. This cytokine stimulates cell proliferationand prevents apoptosis. It functions through the interleukin-9 receptor(IL9R), which activates different signal transducer and activator (STAT)proteins and thus connects this cytokine to various biological processes. Thegene encoding this cytokine has been identified as a candidate gene forasthma.
Source : Hordeum vulgare(barley grain)
Species : Human
Form : Lyophilized from a0.2 µm sterile filtered solution of PBS, pH 7.2.
Molecular Weight : Predicted MW 16.2kDa. The glycosylated recombinant protein migrates as 3 bands with anapparent MW 20, 22 and 24 kDa in SDS-PAGE.
Purity : >95% by SDS-PAGE.Purified product carries no pyrogenic or pro-inflammatory contaminants, asassayed with monocyte activation test using custom human Multiplex CytokineAssay measuring IL-6, TNF-alpha and IL-1beta induction.
Endotoxin Level : < 0.005 ng perµg of product (0.05 EU/µg) as measured by turbidimetric kinetic assay.
Biological Activity : Bioactivity is assayed by measuring the dosedependent effect of human IL9 on proliferation of mouse TS1h9RA3 cell line,transfected with human IL9 receptor. The ED50 for a typical batch of recombinanthuman IL9, is 0.1 ng/ml.
Reconstitution : Centrifuge vial prior to opening. First add steriledistilled water to the vial to fully solubilize the protein to aconcentration not less than 100 µg/ml. After complete solubilization of theprotein, it can be further diluted to other aqueous solutions.
Storage : The lyophilizedprotein, though stable at room temperature for two weeks, is best stored at-20°C. Reconstituted protein should be used immediately or stored in workingaliquots at -20°C. Avoid repeated freeze-thaw cycles.
OfficialSymbol : IL9
Gene Name : IL9 interleukin 9 [ Homosapiens ]
Synonyms : IL9; interleukin 9;P40; HP40; IL-9; interleukin-9; Cytokine P40; cytokine P40; p40 cytokine; OTTHUMP00000159423;T-cell growth factor p40; p40 T-cell and mast cell growth factor; homolog ofmouse T cell and mast cell growth factor 40
Gene ID : 3578
mRNA Refseq : NM_000590
Protein Refseq : NP_000581
MIM : 146931
UniProt ID : P15248
Chromosome Location : 5q31.1
Pathway : Asthma;Cytokine-cytokine receptor interaction; IL-9 Signaling Pathway; Jak-STATsignaling pathway
Function : cytokine activity;growth factor activity; interleukin-9 receptor binding
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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