| Species : |
Human |
| Source : |
Hordeum Vulgare |
| Tag : |
HQ |
| Description : |
Interleukin 9, alsoknown as IL9, is a cytokine (cell signalling molecule) belonging to the groupof interleukins. The protein encoded by this gene is a cytokine produced byT-cells and specifically by CD4+ helper cells that acts as a regulator of avariety of hematopoietic cells. This cytokine stimulates cell proliferationand prevents apoptosis. It functions through the interleukin-9 receptor(IL9R), which activates different signal transducer and activator (STAT)proteins and thus connects this cytokine to various biological processes. Thegene encoding this cytokine has been identified as a candidate gene forasthma. |
| Form : |
Lyophilized from a0.2 µm sterile filtered solution of PBS, pH 7.2. |
| Molecular Weight : |
Predicted MW 16.2kDa. The glycosylated recombinant protein migrates as 3 bands with anapparent MW 20, 22 and 24 kDa in SDS-PAGE. |
| Purity : |
>95% by SDS-PAGE.Purified product carries no pyrogenic or pro-inflammatory contaminants, asassayed with monocyte activation test using custom human Multiplex CytokineAssay measuring IL-6, TNF-alpha and IL-1beta induction. |
| Endotoxin Level : |
< 0.005 ng perµg of product (0.05 EU/µg) as measured by turbidimetric kinetic assay. |
| Biological Activity : |
Bioactivity is assayed by measuring the dosedependent effect of human IL9 on proliferation of mouse TS1h9RA3 cell line,transfected with human IL9 receptor. The ED50 for a typical batch of recombinanthuman IL9, is 0.1 ng/ml. |
| Reconstitution : |
Centrifuge vial prior to opening. First add steriledistilled water to the vial to fully solubilize the protein to aconcentration not less than 100 µg/ml. After complete solubilization of theprotein, it can be further diluted to other aqueous solutions. |
| Storage : |
The lyophilizedprotein, though stable at room temperature for two weeks, is best stored at-20°C. Reconstituted protein should be used immediately or stored in workingaliquots at -20°C. Avoid repeated freeze-thaw cycles. |
| OfficialSymbol : |
IL9 |
