Recombinant Human ITGA4 & ITGB7 protein, His-Avi-tagged, Biotinylated

• Cat.No.: ITGA4 & ITGB7-163H
• Product Overview: Biotinylated Recombinant Human ITGA4 & ITGB7 protein(P13612-1(ITGA4)&P26010-1(ITGB7))(Tyr34-Thr977(ITGA4) & Glu20-His723(ITGB7)) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 34-977aa (ITGA4) & 20-723aa (ITGB7)
• Conjugation/Label: Biotin
• Form: Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Biotinylated Human ITGA4&ITGB7, His-Avi Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-ITGA4 Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 112.5 kDa (ITGA4) and 81.3 kDa (ITGB7). Due to glycosylation, the protein migrates to 68-72 kDa, 78-83 kDa, 110-120 kDa and 130-150 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE
• Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ITGA4 integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) [ Homo sapiens ]
• Official Symbol: ITGA4
• Synonyms: ITGA4; integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor); CD49D; integrin alpha-4; CD49d; 269C wild type; integrin alpha 4; integrin alpha-IV; VLA-4 subunit alpha; integrin alpha-4 subunit; CD49 antigen-like family member D; antigen CD49D, alpha-4 subunit of VLA-4 receptor; very late activation protein 4 receptor, alpha 4 subunit; IA4; MGC90518
• Gene ID: 3676
• mRNA Refseq: NM_000885
• Protein Refseq: NP_000876
• MIM: 192975
• UniProt ID: P13612
• Gene Name 2: ITGB7 integrin, beta 7 [ Homo sapiens ]
• Official Symbol 2: ITGB7
• Synonyms 2: ITGB7; integrin, beta 7; integrin beta-7; integrin beta 7 subunit; gut homing receptor beta subunit
• Gene ID 2: 3695
• mRNA Refseq 2: NM_000889
• Protein Refseq 2: NP_000880
• MIM 2: 147559
• UniProt ID 2: P26010