Recombinant Human ITGA4 & ITGB7 Protein, His-Avi-tagged, Biotinylated

• Cat.No.: ITGA4 & ITGB7-163H
• Product Overview: Recombinant Biotinylated Human ITGA4 & ITGB7 Heterodimer Protein is expressed from HEK293 cells. It contains AA Tyr 34 - Thr 977 (ITGA4) & Glu 20 - His 723 (ITGB7) (Accession # P13612-1 (ITGA4) & P26010-1 (ITGB7)). Subunit ITGA4 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag and subunit ITGB7 contains no tag but a basic tail at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 34-977;20-723 a.a.
• Predicted N Terminal: Tyr 34 (ITGA4) & Glu 20 (ITGB7)
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5, 10% trehalose.
• Molecular Mass: Human ITGA4 & ITGB7 Heterodimer Protein, produced by co-expression of ITGA4 and ITGB7, has a calculated MW of 113.2 kDa (ITGA4) & 81.8 kDa (ITGB7).
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Official Symbol: ITGA4; ITGB7
• Synonyms: Integrin alpha 4 beta 7, ITGA4 & ITGB7