Recombinant Human ITGA6 & ITGB1 Heterodimer Protein, His-tagged

• Cat.No.: ITGA6&ITGB1-359H
• Product Overview: Recombinant Human ITGA6 & ITGB1 Heterodimer Protein, His-tagged contains Phe 24 - Gly 1012 (Integrin alpha 6, P23229-2) and Gln 21 - Asp 728 (Integrin beta 1, P05556-1) was co-expressed in human 293 cells (HEK293) at ACRObiosystems. Predicted N-terminus: Phe 24 (ITGA6) & Gln 21 (ITGB1). Human ITGA6 & ITGB1 Heterodimer Protein, produced by co-expression of ITGA6 and ITGB1, has a calculated MW of 116.8 kDa (ITGA6) and 83.7 kDa (ITGB1). Subunit ITGA6 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The predicted N-terminus is Phe 24 (ITGA6) & Gln 21 (ITGB1). As a result of glycosylation, the protein migrates as 120-130 kDa under reducing (R) condition, and 100-125 kDa and 130-150 kDa under non-reducing (NR) condition (SDS-PAGE).

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Phe 24 - Gly 1012 (ITGA6) & Gln 21 - Asp 728 (ITGB1)
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Molecular Mass: 116.8 kDa (ITGA6) & 83.7 kDa (ITGB1)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: ITGA6 integrin, alpha 6 [ Homo sapiens ] & ITGB1 integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) [ Homo sapiens ]
• Official Symbol: ITGA6 & ITGB1
• Synonyms: ITGA6; integrin, alpha 6; integrin alpha-6; CD49f; VLA-6; ITGA6B; FLJ18737; DKFZp686J01244; & ITGB1; FNRB, MDF2, MSK12; integrin beta-1; CD29; GPIIA; FNRB; MDF2; VLAB; MSK12; VLA-BETA
• Gene ID: 3655 & 3688
• mRNA Refseq: NM_000210 & NM_002211
• Protein Refseq: NP_000201 & NP_002202
• MIM: 147556 & 135630
• UniProt ID: P23229 & P05556