Recombinant Human ITGAV & ITGB3 Protein, His-Avi-tagged, Biotinylated

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Recombinant Human ITGAV & ITGB3 Protein, His-Avi-tagged, Biotinylated

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Cat.No. : ITGAV & ITGB3-738H
Product Overview : Biotinylated Human ITGAV & ITGB3 Heterodimer Protein is expressed from HEK293 cells. It contains AA Phe 31 - Val 992 (ITGAV) & Gly 27 - Asp 718 (ITGB3) (Accession # NP_002201.1 (ITGAV) & P05106-1 (ITGB3)). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag and subunit ITGB3 contains no tag but a basic tail at the C-terminus.
Source : HEK293
Species : Human
Tag : His-Avi
Predicted N Terminal : Phe 31 (ITGAV) & Gly 27 (ITGB3)
Form : Lyophilized from 0.22 um filtered solution in 50 mM Tris, 2 mM CaCl2, 150 mM NaCl, pH7.5, 10% trehalose.
Bio-activity : Immobilized Fibronectin at 5 ug/mL (100 uL/well) can bind Biotinylated Human ITGAV & ITGB3 Heterodimer Protein with a linear range of 0.078-1.25 ug/mL. Immobilized Vitronectin at 5 ug/mL (100 uL/well) can bind Biotinylated Human ITGAV & ITGB3 Heterodimer Protein with a linear range of 0.078-1.25 ug/mL.
Molecular Mass : Human ITGAV & ITGB3 Heterodimer Protein, produced by co-expression of ITGAV and ITGB3, has a calculated MW of 114.7 kDa (ITGAV) and 81.8 kDa (ITGB3). The reducing protein migrates as 120-130 kDa and 145-150 (ITGAV) and 95-100 kDa (ITGB3) respectively due to glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Characteristic : Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Official Symbol : ITGAV; ITGB3
Synonyms : Integrin alpha V beta 3, ITGAV & ITGB3

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