Recombinant Human ITGAV&ITGB6 protein, His-Avi-tagged

• Cat.No.: ITGAV&ITGB6-5633H
• Product Overview: Recombinant Human ITGAV&ITGB6 protein(P06756-1(ITGAV)&P18564-1(ITGB6))(Phe31-Val992(ITGAV) & Gly22-Asn707(ITGB6)), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Phe31-Val992(ITGAV) & Gly22-Asn707(ITGB6)
• Tag: C-His-Avi
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human ITGAV&ITGB6, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 63.3 ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 114 kDa (ITGAV)&79.1 kDa (ITGB6). Due to glycosylation, the protein migrates to 90-150 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• GeneID 2: 3694

Gene Information

• Gene Name: ITGAV integrin, alpha V [ Homo sapiens ]
• Official Symbol: ITGAV
• Synonyms: ITGAV; integrin, alpha V; antigen identified by monoclonal L230 , integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) , MSK8, vitronectin receptor , VNRA, VTNR; integrin alpha-V; CD51; integrin alphaVbeta3; vitronectin receptor subunit alpha; antigen identified by monoclonal L230; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); MSK8; VNRA; VTNR; DKFZp686A08142
• Gene ID: 3685
• mRNA Refseq: NM_001144999
• Protein Refseq: NP_001138471
• MIM: 193210
• UniProt ID: P06756
• Gene Name 2: ITGB6 integrin, beta 6 [ Homo sapiens ]
• Official Symbol 2: ITGB6
• Synonyms 2: ITGB6; integrin, beta 6; integrin beta-6
• mRNA Refseq 2: NM_000888
• Protein Refseq 2: NP_000879
• MIM 2: 147558
• UniProt ID 2: P18564