Recombinant Human ITGAV&ITGB8 protein, His-Avi-tagged

• Cat.No.: ITGAV?& ITGB8-3117H
• Product Overview: Recombinant Human ITGAV&ITGB8 protein(P06756-1(ITGAV)&P26012-1(ITGB8))(Phe31-Val992(ITGAV) & Glu43-Arg684(ITGB8)), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Phe31-Val992(ITGAV) & Glu43-Arg684(ITGB8)
• Tag: C-His-Avi
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human ITGAV&ITGB8, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 0.85 μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 114 kDa (ITGAV)&76 kDa (ITGB8). Due to glycosylation, the protein migrates to 90-150 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• GeneID 2: 3696

Gene Information

• Gene Name: ITGAV integrin, alpha V [ Homo sapiens ]
• Official Symbol: ITGAV
• Synonyms: ITGAV; integrin, alpha V; antigen identified by monoclonal L230 , integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) , MSK8, vitronectin receptor , VNRA, VTNR; integrin alpha-V; CD51; integrin alphaVbeta3; vitronectin receptor subunit alpha; antigen identified by monoclonal L230; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); MSK8; VNRA; VTNR; DKFZp686A08142
• Gene ID: 3685
• mRNA Refseq: NM_001144999
• Protein Refseq: NP_001138471
• MIM: 193210
• UniProt ID: P06756
• Gene Name 2: ITGB8 integrin, beta 8 [ Homo sapiens ]
• Official Symbol 2: ITGB8
• Synonyms 2: ITGB8; integrin, beta 8; integrin beta-8
• mRNA Refseq 2: NM_002214
• Protein Refseq 2: NP_002205
• MIM 2: 604160
• UniProt ID 2: P26012