Recombinant Human ITGAX&ITGB2 protein, His-tagged

• Cat.No.: ITGAX&ITGB2-1599H
• Product Overview: Recombinant Human ITGAX&ITGB2 protein(Phe20-Pro1107(ITGAX)&Gln23-Asn700(ITGB2)), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Molecular Mass: Molecular CharacterizationHuman ITGAX&ITGB2 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAX and ITGB2, has a calculated MW of 126.5 kDa (ITGAX) and 80.2 kDa (ITGB2). Subunit ITGAX is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB2 contains no tag but a basic tail at the C-terminus. The protein migrates as 145 kDa (ITGAX) and 90 kDa (ITGB2) under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Phe20-Pro1107(ITGAX)&Gln23-Asn700(ITGB2)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ITGAX integrin, alpha X (complement component 3 receptor 4 subunit) [ Homo sapiens ]
• Official Symbol: ITGAX
• Synonyms: ITGAX; integrin, alpha X (complement component 3 receptor 4 subunit); CD11C, integrin, alpha X (antigen CD11C (p150), alpha polypeptide); integrin alpha-X; CD11c; leu M5, alpha subunit; p150 95 integrin alpha chain; CD11 antigen-like family member C; leukocyte adhesion receptor p150,95; myeloid membrane antigen, alpha subunit; leukocyte surface antigen p150,95, alpha subunit; leukocyte adhesion glycoprotein p150,95 alpha chain; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); CD11C; SLEB6
• Gene ID: 3687
• mRNA Refseq: NM_000887
• Protein Refseq: NP_000878
• MIM: 151510
• UniProt ID: P20702