Recombinant Human KDR Protein, His-Avi-tagged, Biotinylated

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Recombinant Human KDR Protein, His-Avi-tagged, Biotinylated

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Cat.No. : KDR-632H
Product Overview : Biotinylated Human VEGF R2, His Tag is expressed from HEK293 cells. It contains AA Ala 20 - Glu 764 (Accession # AAI31823). This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag.
Source : HEK293
Species : Human
Tag : His-Avi
Predicted N Terminal : Ala 20
Form : Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Bio-activity : Immobilized Human VEGF165, Tag Free at 2 ug/mL (100 uL/well) can bind Biotinylated Human VEGF R2, His Tag with a linear range of 0.15-2.5 ug/mL.
Molecular Mass : The protein has a calculated MW of 86 kDa. The protein migrates as 120-130 kDa under reducing condition (SDS-PAGE) due to glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >95% as determined by SDS-PAGE..
Characteristic : Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : KDR kinase insert domain receptor (a type III receptor tyrosine kinase) [ Homo sapiens ]
Official Symbol : KDR
Synonyms : KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); vascular endothelial growth factor receptor 2; CD309; FLK1; VEGFR; VEGFR2; soluble VEGFR2; fetal liver kinase 1; fetal liver kinase-1; protein-tyrosine kinase receptor Flk-1; tyrosine kinase growth factor receptor;
Gene ID : 3791
mRNA Refseq : NM_002253
Protein Refseq : NP_002244
MIM : 191306
UniProt ID : P35968

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