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Recombinant Human KDR Protein, His-Avi-tagged, Biotinylated
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Cat.No. : | KDR-632H |
Product Overview : | Biotinylated Human VEGF R2, His Tag is expressed from HEK293 cells. It contains AA Ala 20 - Glu 764 (Accession # AAI31823). This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag. |
Source : | HEK293 |
Species : | Human |
Tag : | His-Avi |
Predicted N Terminal : | Ala 20 |
Form : | Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose. |
Bio-activity : | Immobilized Human VEGF165, Tag Free at 2 ug/mL (100 uL/well) can bind Biotinylated Human VEGF R2, His Tag with a linear range of 0.15-2.5 ug/mL. |
Molecular Mass : | The protein has a calculated MW of 86 kDa. The protein migrates as 120-130 kDa under reducing condition (SDS-PAGE) due to glycosylation. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method. |
Purity : | >95% as determined by SDS-PAGE.. |
Characteristic : | Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. |
Stability : | Samples are stable for up to twelve months from date of receipt at -70ºC. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents. |
Gene Name : | KDR kinase insert domain receptor (a type III receptor tyrosine kinase) [ Homo sapiens ] |
Official Symbol : | KDR |
Synonyms : | KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); vascular endothelial growth factor receptor 2; CD309; FLK1; VEGFR; VEGFR2; soluble VEGFR2; fetal liver kinase 1; fetal liver kinase-1; protein-tyrosine kinase receptor Flk-1; tyrosine kinase growth factor receptor; |
Gene ID : | 3791 |
mRNA Refseq : | NM_002253 |
Protein Refseq : | NP_002244 |
MIM : | 191306 |
UniProt ID : | P35968 |
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KDR-643HP | Recombinant Human KDR protein, Fc-tagged, R-PE labeled | +Inquiry |
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◆ Lysates
KDR-1520MCL | Recombinant Mouse KDR cell lysate | +Inquiry |
KDR-417HCL | Recombinant Human KDR cell lysate | +Inquiry |
KDR-1769HCL | Recombinant Human KDR cell lysate | +Inquiry |
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◆ Assay kits
Kit-1609 | KDR Activity Assay Kit | +Inquiry |
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