| Description : |
Kelch-like ECH-associated protein 1, also known as cytosolic inhibitor of Nrf2, Kelch-like protein 19, KEAP1 and INRF2, is a cytoplasm and nucleus protein which contains one BACK (BTB/Kelch associated) domain, one BTB (POZ) domain and six Kelch repeats. KEAP1/INRF2 is broadly expressed, with highest levels in skeletal muscle. KEAP1/INRF2 is a key regulator of the NRF2 transcription factor, which transactivates the antioxidant response element (ARE) and upregulates numerous proteins involved in antioxidant defense. Under basal conditions, KEAP1/INRF2 targets NRF2 for ubiquitination and proteolytic degradation and as such is responsible for the rapid turnover of NRF2. KEAP1/INRF2 retains NFE2L2/NRF2 in the cytosol. KEAP1/INRF2 functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. It targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. KEAP1/INRF2 may also retain BPTF in the cytosol. It targets PGAM5 for ubiquitination and degradation by the proteasome. |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
His |
| Molecular Mass : |
20.36 kDa |
| Protein Length : |
Ala90-Ile250 |
| Purity : |
>90 % as determined by SDS-PAGE. |
| Notes : |
For research use only. |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 centigrade for one week. Store at -20 to -80 centigrade for twelve months from the date of receipt. |
| Storage Buffer : |
Supplied as solution form in PBS pH 7.5 or lyophilized from PBS pH 7.5. |
| Reconstitution : |
Reconstitute in sterile water for a stock solution. |
| Shipping : |
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
