Recombinant Human KIT, Fc Chimera

• Cat.No.: KIT-148H
• Product Overview: Recombinant Human stem cell factor receptor was fused to the Fc region of human IgG1 (aa 93-330). The chimeric protein was expressed in modifiedhuman 293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Description: Stem cell factor receptor (SC R), also known as c-kit, has a molecular weight of 145 kDa as a mature protein and is a member of the type III receptor tyrosine kinase (RTK) family that includes platelet-derived growth factor (PDGF) receptors and the macrophage colony-stimulating factor 1 (CSF-1) (c-fms) receptor. SCFR is essential for the development of normal hematopoietic cells and plays an important role in the survival, proliferation, and differentiation of mast cells, melanocytes, and germ cells. SCF R contains five extracellular immunoglobulin-like (Ig) domains, a single juxtamembrane domain (JMD), a cytoplasmic tyrosine kinase domain,Binding of SCF R to its ligand (stem cell factor; SCF) induces receptor dimerization, trans autophosphorylation of the kinase domain, recruitment of signaling proteins via phosphotyrosine binding or Src homology 2 (SH2) domains.
• Molecular Mass: SCFR-Fc Chimera migrates as a broad band between 90 and 150 kDa in SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified SCFR -Fc Chimera that has a predicted molecular mass of 83.1 kDa.
• PI: SCFR-Fc Chimera separates into a number of isoforms with a pI between 4.5 and 8 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified SCF R – Fc Chimera that has a predicted pI of 6.6.
• % Carbohydrate: purified SCFR - Fc Chimera consists of 5-45% carbohydrate by weight.
• Glycosylation: SCFR - FcB Chimera is N- and O-glycosylated.
• Purity: >95%, as determined by SDS-PAGE and visualized by silver stain.
• Formulation: When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
• Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
• Storage: Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C.
• Activity: The ED50 of the SCF R - Fc Chimera typically 1-3 ug/ml as measured by its ability to neutralize SCF mediated proliferation using the human growth factor dependent M-07e cell line.

Gene Information

• Gene Name: KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog [ Homo sapiens ]
• Synonyms: v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; PBT; SCFR; C-Kit; CD117; KIT; soluble KIT variant 1; mast/stem cell growth factor receptor; proto-oncogene tyrosine-protein kinase Kit; piebald trait; EC 2.7.10.1; c-kit; CD117 antigen; Proto-oncogene tyrosine-protein kinase Kit
• Gene ID: 3815
• mRNA Refseq: NM_000222
• Protein Refseq: NP_000213
• MIM: 164920
• UniProt ID: P10721
• Chromosome Location: 4q11-q12
• Pathway: Cytokine-cytokine receptor interaction; Melanogenesis; Pathways in cancer; Acute myeloid leukemia; Endocytosis; Hematopoietic cell lineage
• Function: ATP binding; nucleotide binding; protein binding; receptor activity; stem cell factor receptor activity; transferase activity; receptor signaling protein tyrosine kinase activity