Recombinant Human KIT Protein, His-tagged, Alexa Fluor 555 conjugated

• Cat.No.: KIT-396HAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant human KIT (P10721-1) (Val50-Gln190), fused with a polyhistidine tag at the N-terminus, was produced in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized
• Molecular Mass: The recombinant human KIT consists of 156 amino acids and predicts a molecular mass of 17.9 kDa. It migrates as an approximately 18 kDa band in SDS-PAGE under reducing conditions.
• Protein length: 156
• N-terminal Sequence Analysis: His
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile 137 mM NaCl.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog [ Homo sapiens ]
• Official Symbol: KIT
• Gene ID: 3815
• mRNA Refseq: NM_000222
• Protein Refseq: NP_000213
• MIM: 164920
• UniProt ID: P10721