Recombinant Human KIT protein(Met1-Thr516), hFc-tagged

• Cat.No.: KIT-324H
• Product Overview: Recombinant Human KIT (P10721-2) (Met1-Thr516) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: C-hFc
• Protein length: Met1-Thr516
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Human SCF His at 2 μg/ml (100 μl/well) can bind Human c-Kit hFc, the EC50 of Human c-Kit hFc is 20-60 ng/mL.
• Molecular Mass: The recombinant human KIT/Fc comprises 729 amino acids and has a predicted molecular mass of 82 kDa. The apparent molecular mass of the protein is approximately 93-115 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog [ Homo sapiens ]
• Official Symbol: KIT
• Synonyms: KIT; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; PBT, piebald trait; mast/stem cell growth factor receptor Kit; C Kit; CD117; SCFR; p145 c-kit; proto-oncogene c-Kit; piebald trait protein; soluble KIT variant 1; tyrosine-protein kinase Kit; proto-oncogene tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein; PBT; C-Kit
• Gene ID: 3815
• mRNA Refseq: NM_000222
• Protein Refseq: NP_000213
• MIM: 164920
• UniProt ID: P10721

Reviews

11/28/2020

The quality control measures are evident in the performance of this product.

04/02/2019

Exceptional service!

07/19/2018

Rapid delivery! Ordered and received the product within a short timeframe, allowing us to proceed with our experiments without delays.

Q&As

How does KIT gene expression vary in different tissues, and what factors contribute to tissue-specific regulation?

KIT expression varies across tissues due to tissue-specific transcription factors, epigenetic modifications, and microenvironmental cues influencing its regulation.

How is the expression of the KIT gene regulated at the transcriptional level?

Transcription factors like PU.1 and GATA-2, along with epigenetic modifications, regulate KIT gene expression.

Contribution of KIT gene to germ cell maintenance in the testes and ovaries?

KIT is crucial for germ cell survival and proliferation; mutations may lead to infertility.

What is the role of KIT gene in stem cell biology and regenerative medicine?

KIT maintains and influences stem cell behavior, holding potential in regenerative medicine.

Role of KIT gene in hematopoiesis and implications in hematological disorders?

KIT is vital in hematopoietic stem cell development; mutations link to leukemia and mastocytosis.