Active Recombinant Human KITLG protein, His&hFc-tagged

Cat.No. : KITLG-4990H
Product Overview : Active Recombinant Human KITLG protein(P21583)(26~189 aa), fused with N-terminal His tag and C-terminal hFc tag, was expressed in HEK293.
  • Specification
  • Gene Information
  • Related Products
  • Download
Species : Human
Source : HEK293
Tag : Fc&His
Protein Length : 26-189 aa
Form : Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Bio-activity : Stem cell factor (SCF), also known as mast cell growth factor (MGF), and steel factor (SLF), plays an important role in hematopoiesis, spermatogenesis and melanogenesis. SCF has been shown to stimulate the proliferation of TF-1 cells. To test this effect, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 2 x 10^4 cells/well and incubated for 72h in the presence or absence of various concentrations of SCF at 37°CThe growth of cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450 nm using a microplate reader after incubating the plate for 1-4 hours at 37°C. Cell proliferation of TF-1 cells after incubation with SCF for 72h observed by inverted microscope was shown. The dose-effect curve of SCF was shown. It was obvious that it significantly promoted cell proliferation of TF-1 cells. The ED50 for this effect is typically 3.9 ng/ml.
Molecular Mass : 49.5kDa
Endotoxin : <1.0EU per 1μg (determined by the LAL method).
Purity : > 90% as determined by SDS-PAGE.
Storage : Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
Reconstitution : Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name KITLG KIT ligand [ Homo sapiens ]
Official Symbol KITLG
Synonyms KITLG; KIT ligand; MGF; kit ligand; familial progressive hyperpigmentation 2; FPH2; Kitl; KL 1; mast cell growth factor; SCF; SF; steel factor; stem cell factor; c-Kit ligand; KL-1; SHEP7; kit-ligand; DKFZp686F2250;
Gene ID 4254
mRNA Refseq NM_000899
Protein Refseq NP_000890
MIM 184745
UniProt ID P21583

Not For Human Consumption!

Inquiry

  • Reviews (0)
  • Q&As (0)

Customer Reviews

Write a review

Ask a Question for All KITLG Products

Required fields are marked with *

My Review for All KITLG Products

Required fields are marked with *

0
cart-icon
0
compare icon