Recombinant Human KLF1 protein, His-B2M-tagged

• Cat.No.: KLF1-395H
• Product Overview: Recombinant Human KLF1 protein(NP_006554.1)(1-362aa) fused with two N-terminal tag, His tag and B2M tag, was expressed in E. coli.

Specification

• Description: This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype.
• Source: E. coli
• Species: Human
• Tag: His-B2M
• Form: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
  If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Molecular Mass: 52.2 kDa
• Protein length: 1-362aa
• AA Sequence: MATAETALPSISTLTALGPFPDTQD DFLKWWRSEEAQDMGPGPPDPTEPP LHVKSEDQPGEEEDDERGADATWDL DLLLTNFSGPEPGGAPQTCALAPSE ASGAQYPPPPETLGAYAGGPGLVAG LLGSEDHSGWVRPALRARAPDAFVG PALAPAPAPEPKALALQPVYPGPGA GSSGGYFPRTGLSVPAASGAPYGLL SGYPAMYPAPQYQGHFQLFRGLQGP APGPATSPSFLSCLGPGTVGTGLGG TAEDPGVIAETAPSKRGRRSWARKR QAAHTCAHPGCGKSYTKSSHLKAHL RTHTGEKPYACTWEGCGWRFARSDE LTRHYRKHTGQRPFRCQLCPRAFSR SDHLALHMKRHL
• Purity: Greater than 85% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Gene Information

• Gene Name: KLF1 Kruppel like factor 1 [ Homo sapiens (human) ]
• Official Symbol: KLF1
• Synonyms: CDAN4; EKLF; EKLF PEN
• Gene ID: 10661
• mRNA Refseq: NM_006563.5
• Protein Refseq: NP_006554.1
• MIM: 600599
• UniProt ID: Q13351