Recombinant Human KPNA2 Protein, GST-tagged

• Cat.No.: KPNA2-4897H
• Product Overview: Human KPNA2 full-length ORF ( AAH05978.1, 1 a.a. - 529 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination [provided by RefSeq
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 83.93 kDa
• AA Sequence: MSTNENANTPAARLHRFKNKGKDST EMRRRRIEVNVELRKAKKDDQMLKR RNVSSFPDDATSPLQENRNNQGTVN WSVDDIVKGINSSNVENQLQATQAA RKLLSREKQPPIDNIIRAGLIPKFV SFLGRTDCSPIQFESAWALTNIASG TSEQTKVVVDGGAIPAFISLLASPH AHISEQAVWALGNIAGDGSVFRDLV IKYGAVDPLLALLAVPDMSSLACGY LRNLTWTLSNLCRNKNPAPPIDAVE QILPTLVRLLHHDDPEVLADTCWAI SYLTDGPNERIGMVVKTGVVPQLVK LLGASELPIVTPALRAIGNIVTGTD EQTQVVIDAGALAVFPSLLTNPKTN IQKEATWTMSNITAGRQDQIQQVVN HGLVPFLVSVLSKADFKTQKEAVWA VTNYTSGGTVEQIVYLVHCGIIEPL MNLLTAKDTKIILVILDAISNIFQA AENLGETEKLSIMIEECGGLDKIEA LQNHENESVYKASLSLIEKYFSVEE EEDQNVVPETTSEGYTFQVQDGAPG TFNF
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: KPNA2 karyopherin alpha 2 (RAG cohort 1, importin alpha 1) [ Homo sapiens ]
• Official Symbol: KPNA2
• Synonyms: KPNA2; karyopherin alpha 2 (RAG cohort 1, importin alpha 1); RCH1; importin subunit alpha-2; IPOA1; QIP2; SRP1alpha; pendulin; SRP1-alpha; RAG cohort 1; importin alpha 1; importin alpha 2; importin-alpha-P1; RAG cohort protein 1; karyopherin subunit alpha-2
• Gene ID: 3838
• mRNA Refseq: NM_002266
• Protein Refseq: NP_002257
• MIM: 600685
• UniProt ID: P52292