Recombinant Full Length Human KPNA2 Protein, C-Flag-tagged

• Cat.No.: KPNA2-969HFL
• Product Overview: Recombinant Full Length Human KPNA2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 57.7 kDa
• AA Sequence: MSTNENANTPAARLHRFKNKGKDST EMRRRRIEVNVELRKAKKDDQMLKR RNVSSFPDDATSPLQENRNN QGTVNWSVDDIVKGINSSNVENQLQ ATQAARKLLSREKQPPIDNIIRAGL IPKFVSFLGRTDCSPIQFES AWALTNIASGTSEQTKAVVDGGAIP AFISLLASPHAHISEQAVWALGNIA GDGSVFRDLVIKYGAVDPLL ALLAVPEMSSLACGYLRNLTWTLSN LCRNKNPAPPIDAVEQILPTLVRLL HHDDPEVLADTCWAISYLTD GPNERIGMVVKTGVVPQLVKLLGAS ELPIVTPALRAIGNIVTGTDEQTQV VIDAGALAVFPSLLTNPKTN IQKEATWTMSNITAGRQDQIQQVVN HGLVPFLVSVLSKADFKTQKEAVWA VTNYTSGGTVEQIVYLVHCG IIEPLMNLLTAKDTKIILVILDAIS NIFQAAEKLGETEKLSIMIEECGGL DKIEALQNHENESVYKASLS
  LIEKYFSVEEEEDQNVVPETTSEGY TFQVQDGAPGTFNFTRTRPLEQKLI SEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Stem cell - Pluripotency

Gene Information

• Gene Name: KPNA2 karyopherin subunit alpha 2 [ Homo sapiens (human) ]
• Official Symbol: KPNA2
• Synonyms: QIP2; RCH1; IPOA1; PTAC58; SRP1alpha; SRP1-alpha
• Gene ID: 3838
• mRNA Refseq: NM_002266.4
• Protein Refseq: NP_002257.1
• MIM: 600685
• UniProt ID: P52292