Recombinant Human KRAS protein(G12D, Q61H), His-tagged

• Cat.No.: KRAS-458H
• Product Overview: A DNA sequence encoding the human KRAS (P01116-2)(Thr2-Cys185, with natural variant Gly 12 Asp and Gln 61 His) was expressed with a polyhistide tag at the N-terminus.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Thr2-Cys185
• Predicted N Terminal: Met
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human KRAS consists of 202 amino acids and has a calculated molecular mass of 23.3 kDa.
• Endotoxin: Please contact us for more information.
• Purity: > 85 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
• Storage: Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: KRAS KRAS proto-oncogene, GTPase [ Homo sapiens (human) ]
• Official Symbol: KRAS
• Synonyms: C-K-RAS; CFC2; K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI- RAS; KRAS1; KRAS2; NS; NS3; RALD; RASK2
• Gene ID: 3845
• mRNA Refseq: NM_001369786.1
• Protein Refseq: NP_001356715.1
• MIM: 190070
• UniProt ID: P01116