Recombinant Human KRAS Protein, His-tagged, Alexa Fluor 555 conjugated

• Cat.No.: KRAS-3965HAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant human KRAS (AAH13572.1) (Thr 2-Cys 185), fused with a polyhistide tag at the N-terminus, was produced in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized
• Molecular Mass: The recombinant human KRAS consisting of 195 amino acids and has a calculated molecular mass of 22.5 kDa. It migrates as an 25 kDa band in SDS-PAGE under reducing conditions as predicted
• Protein length: 195
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile 20 mM Tris, 0.5M NaCl, pH 7.5
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog [ Homo sapiens ]
• Official Symbol: KRAS
• Gene ID: 3845
• mRNA Refseq: NM_004985
• Protein Refseq: NP_004976
• MIM: 190070
• UniProt ID: P01116