Recombinant Human KRT15 293 Cell Lysate KRT15-4879HCL

Recombinant Human KRT15 293 Cell Lysate

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Recombinant Human KRT15 293 Cell Lysate

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Cat.No. : KRT15-4879HCL Optional Service: Optional requirements on this protein
Description : Antigen standard for keratin 15 (KRT15) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : KRT15 keratin 15 [ Homo sapiens ]
Official Symbol : KRT15
Synonyms : KRT15; keratin 15; keratin, type I cytoskeletal 15; CK15; cytokeratin 15; K1CO; K15; basic; beta; type I cytoskeletal 15; CK-15; cytokeratin-15; keratin-15, beta; keratin-15, basic;
Gene ID : 3866
mRNA Refseq : NM_002275
Protein Refseq : NP_002266
MIM : 148030
UniProt ID : P19012
Chromosome Location : 17q21-q23
Function : protein binding; structural constituent of cytoskeleton;
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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