Recombinant Human KRT5 protein, His & S-tagged

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Recombinant Human KRT5 protein, His & S-tagged

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Cat.No. : KRT5-2691H
Product Overview : Recombinant Human KRT5 aa. (Thr166~Leu474 (Accession # P13647)) fused with N-terminal His & S tag was produced in E. coli cells.
Description : The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
Source : E. coli
Species : Human
Tag : His & S
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 42.0kDa
Protein length : Thr166~Leu474 (Accession # P13647)
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : >95%
Characteristic : The isoelectric point is 5.2.
Applications : SDS-PAGE; WB; ELISA; IP
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage buffer : Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative.
Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4.
SDS-PAGE :

Gene Name : KRT5 keratin 5 [ Homo sapiens (human) ]
Official Symbol : KRT5
Synonyms : K5; CK5; DDD; DDD1; EBS2; KRT5A; 58 kda cytokeratin; CK-5; cytokeratin-5; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; type-II keratin Kb5; keratin, type II cytoskeletal 5
Gene ID : 3852
mRNA Refseq : NM_000424.3
Protein Refseq : NP_000415.2
UniProt ID : P13647
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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