Recombinant Human Kruppel-like factor 12, His-tagged

• Cat.No.: KLF12-602H
• Product Overview: KLF12, 1-402aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-402 a.a.
• Description: KLF12, as known as Krueppel-like factor 12, is a member of the Kruppel-like zinc finger protein family and can repress expression of the AP-2 alpha gene by binding to a specific site in the AP-2 alpha gene promoter. Activator protein-2 alpha (AP-2 alpha) is a developmentally-regulated transcription factor and important regulator of gene expression during vertebrate development and carcinogenesis. Repression by the encoded protein requires binding with a corepressor, CtBP1. Two transcript variants encoding different isoforms have been found for this gene.cers. Recombinant human KLF12 protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.4M Urea
• Molecular Mass: 46.6 kDa(425aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNIHMKR KTIKNINTFE NRMLMLDGMP AVRVKTELLE SEQGSPNVHN YPDMEAVPLL LNNVKGEPPE DSLSVDHFQT QTEPVDLSIN KARTSPTAVS SSPVSMTASA SSPSSTSTSS SSSSRLASSP TVITSVSSAS SSSTVLTPGP LVASASGVGG QQFLHIIHPV PPSSPMNLQS NKLSHVHRIP VVVQSVPVVY TAVRSPGNVN NTIVVPLLED GRGHGKAQMD PRGLSPRQSK SDSDDDDLPN VTLDSVNETG STALSIARAV QEVHPSPVSR VRGNRMNNQK FPCSISPFSI ESTRRQRRSE SPDSRKRRIH RCDFEGCNKV YTKSSHLKAH RRTHTGEKPY KCTWEGCTWK FARSDELTRH YRKHTGVKPF KCADCDRSFS RSDHLALHRR RHMLV
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: KLF12 Kruppel-like factor 12 [ Homo sapiens ]
• Official Symbol: KLF12
• Synonyms: KLF12; Kruppel-like factor 12; Krueppel-like factor 12; AP 2 repressor; AP 2rep; AP 2rep transcription factor; AP2REP; HSPC122; KLF12 zinc finger transcriptional repressor; AP-2 repressor; AP-2rep transcription factor; transcriptional repressor AP-2rep; AP-2rep
• Gene ID: 11278
• mRNA Refseq: NM_007249
• Protein Refseq: NP_009180
• MIM: 607531
• UniProt ID: Q9Y4X4
• Chromosome Location: 13q22
• Function: DNA binding; metal ion binding; sequence-specific DNA binding transcription factor activity; transcription corepressor activity; zinc ion binding