Recombinant Human Lactate Dehydrogenase B, His-tagged

• Cat.No.: LDHB-2570H
• Product Overview: Recombinant human LDHB protein, fused to His-tagging at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW = 38.8 kDa (354aa).

Specification

• Cat. No.: LDHB-2570H
• Description: LDHB, also known as L-lactate dehydrogenase B chain, is a member of the lactate dehydrogenase family. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As this protein can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). The LDH family consists of three members, LDH-A, LDH-B and LDH-C. LDHs function as powerful markers for germ cell tumors.
• Source: E.coli.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MATLKEKLIA PVAEEEATVP NNKITVVGVG QVGMACAISI LGKSLADELA LVDVLEDKLK GEMMDLQHGS LFLQTPKIVA DKDYSVTANS KIVVVTAGVR QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCIIIVVSNP VDILTYVTWK LSGLPKHRVI GSGCNLDSAR FRYLMAEKLG IHPSSCHGWI LGEHGDSSVA VWSGVNVAGV SLQELNPEMG TDNDSENWKE VHKMVVESAY EVIKLKGYTN WAIGLSVADL IESMLKNLSR IHPVSTMVKG MYGIENEVFL SLPCILNARG LTSVINQKLK DDEVAQLKKS ADTLWDIQKD LKDL
• Form: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol 1mM DTT.
• Molecular Weight: 38.8 kDa (354aa) confirmed by MALDI-TOF
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Biological Activity: Specific activity is > 6 units/mg. in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37°C.
• Activity Assay: 1. Prepare a 1.5 ml reaction mix into a suitable container, adjust to pH7.5 at 37C, and pre-chill on ice before use: The final concentrations are 100mM Sodium phosphate, 0.12 mM beta-nicotinamide adenine dinucleotide, reduced form, 2.3mM Pyruvate, 0.033% (w/v) bovine serum albumin. 2. Equilibrate to 37C and monitor at A340nm until the value is constant using a spectrophotometer3. Add 50ul of recombinant LDHB protein in various concentrations (0.1ug, 0.5ug) in assay buffer. 4. Mix by inversion and record the increase at A340nm for 5 minutes.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: LDHB lactate dehydrogenase B [ Homo sapiens ]
• Synonyms: LDHB; lactate dehydrogenase B; LDH-H; TRG-5; L-lactate dehydrogenase B chain; LDH-B; LDH heart subunit; OTTHUMP00000165231; renal carcinoma antigen NY-REN-46; EC 1.1.1.27
• Gene ID: 3945
• mRNA Refseq: NM_001174097
• Protein Refseq: NP_001167568
• MIM: 150100
• UniProt ID: P07195
• Chromosome Location: 12p12.2-p12.1
• Function: L-lactate dehydrogenase activity; NAD or NADH binding; identical protein binding; oxidoreductase activity

Reviews

04/27/2022

I highly endorse the utilization of the LDHB Protein for researchers seeking a superior protein solution that not only meets their experimental needs but also benefits from the exceptional technical support provided by the manufacturer.

11/05/2020

Their expertise has proven instrumental in overcoming obstacles and has greatly contributed to the success of my research endeavors.

10/14/2016

Their team of experts has been extremely helpful in addressing any concerns or queries I encountered during my experiments.

Q&As

Does LDHB play a role in heart disease?

Yes, LDHB dysregulation is associated with heart conditions like myocardial infarction (heart attack) and heart failure.

Are there specific populations more susceptible to LDHB-related conditions?

While LDHB-related diseases can affect anyone, some genetic predispositions may heighten the risk in certain ethnic groups.

How is LDHB protein clinically relevant?

It plays a role in various physiological processes and has implications in disease states.

How does LDHB contribute to cancer?

In certain cancers, elevated LDHB levels promote tumor growth by supporting the Warburg effect, where cancer cells use glycolysis even in the presence of oxygen.

Can LDHB be a therapeutic target in cancer treatment?

Targeting LDHB shows promise in inhibiting cancer growth, and there's ongoing research in developing drugs that target this enzyme.