Recombinant Human LCMT2 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: LCMT2-6269H
• Product Overview: LCMT2 MS Standard C13 and N15-labeled recombinant protein (NP_055608) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this intronless gene belongs to the highly variable methyltransferase superfamily. This gene is the inferred homolog of the Saccharomyces cerevisiae carboxymethyltransferase gene PPM2 that is essential for the synthesis of the hypermodified guanosine Wybutosine (yW).
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 75.6 kDa
• AA Sequence: MGPRSRERRAGAVQNTNDSSALSKR SLAARGYVQDPFAALLVPGAARRAP LIHRGYYVRARAVRHCVRAFLEQIG APQAALRAQILSLGAGFDSLYFRLK TAGRLARAAVWEVDFPDVARRKAER IGETPELCALTGPFERGEPASALCF ESADYCILGLDLRQLQRVEEALGAA GLDAASPTLLLAEAVLTYLEPESAA ALIAWAAQRFPNALFVVYEQMRPQD AFGQFMLQHFRQLNSPLHGLERFPD VEAQRRRFLQAGWTACGAVDMNEFY HCFLPAEERRRVENIEPFDEFEEWH LKCAHYFILAASRGDTLSHTLVFPS SEAFPRVNPASPSGVFPASVVSSEG QVPNLKRYGHASVFLSPDVILSAGG FGEQEGRHCRVSQFHLLSRDCDSEW KGSQIGSCGTGVQWDGRLYHTMTRL SESRVLVLGGRLSPVSPALGVLQLH FFKSEDNNTEDLKVTITKAGRKDDS TLCCWRHSTTEVSCQNQEYLFVYGG RSVVEPVLSDWHFLHVGTMAWVRIP VEGEVPEARHSHSACTWQGGALIAG GLGASEEPLNSVLFLRPISCGFLWE SVDIQPPITPRYSHTAHVLNGKLLL VGGIWIHSSSFPGVTVINLTTGLSS EYQIDTTYVPWPLMLHNHTSILLPE EQQLLLLGGGGNCFSFGTYFNPHTV TLDLSSLSAGQTRTRPLEQKLISEE DLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: LCMT2 leucine carboxyl methyltransferase 2 [ Homo sapiens (human) ]
• Official Symbol: LCMT2
• Synonyms: LCMT2; leucine carboxyl methyltransferase 2; KIAA0547; MGC9534; PPM2; tRNA yW synthesizing protein 4; TYW4; tRNA-yW synthesizing protein 4; tRNA-Wybutosine-synthesizing protein 4; p21WAF1/CIP1 promoter-interacting protein; FLJ51321
• Gene ID: 9836
• mRNA Refseq: NM_014793
• Protein Refseq: NP_055608
• MIM: 611246
• UniProt ID: O60294