Active Recombinant Human LCN2 protein, hFc-tagged, Low Endotoxin

• Cat.No.: LCN2-7864H
• Product Overview: Recombinant Human LCN2 protein(P80188-1)(Gln21-Gly198), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Protein Length: 21-198 aa
• Form: Lyophilized from 0.22μm filtered solution in 50mM MES,150mM NaCl (pH 6.5). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human NGAL, hFc Tag at 1 μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-NGAL Antibody, hFc Tag with the EC50 21.4ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 47.2 kDa. Due to glycosylation, the protein migrates to 48-52 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: LCN2 lipocalin 2 [ Homo sapiens ]
• Official Symbol: LCN2
• Synonyms: LCN2; lipocalin 2; lipocalin 2 (oncogene 24p3); neutrophil gelatinase-associated lipocalin; 24p3; neutrophil gelatinase associated lipocalin; NGAL; oncogene 24p3; siderocalin; p25; lipocalin-2; migration-stimulating factor inhibitor; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; MSFI
• Gene ID: 3934
• mRNA Refseq: NM_005564
• Protein Refseq: NP_005555
• MIM: 600181
• UniProt ID: P80188