Description : |
Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues. |
Source : |
HEK293 |
Species : |
Human |
Tag : |
Myc/DDK |
Molecular Mass : |
70.3 kDa |
AA Sequence : |
MARGSVSDEEMMELREAFAKVDTDG NGYISFNELNDLFKAACLPLPGYRV REITENLMATGDLDQDGRISFDEFI KIFHGLKSTDVAKTFRKAINKKEGI CAIGGTSEQSSVGTQHSYSEEEKYA FVNWINKALENDPDCRHVIPMNPNT NDLFNAVGDGIVLCKMINLSVPDTI DERTINKKKLTPFTIQENLNLALNS ASAIGCHVVNIGAEDLKEGKPYLVL GLLWQVIKIGLFADIELSRNEALIA LLREGESLEDLMKLSPEELLLRWAN YHLENAGCNKIGNFSTDIKDSKAYY HLLEQVAPKGDEEGVPAVVIDMSGL REKDDIQRAECMLQQAERLGCRQFV TATDVVRGNPKLNLAFIANLFNRYP ALHKPENQDIDWGALEGETREERTF RNWMNSLGVNPRVNHLYSDLSDALV IFQLYEKIKVPVDWNRVNKPPYPKL GGNMKKLENCNYAVELGKNQAKFSL VGIGGQDLNEGNRTLTLALIWQLMR RYTLNILEEIGGGQKVNDDIIVNWV NETLREAEKSSSISSFKDPKISTSL PVLDLIDAIQPGSINYDLLKTENLN DDEKLNNAKYAISMARKIGARVYAL PEDLVEVNPKMVMTVFACLMGKGMK RVTRTRPLEQKLISEEDLAANDILD YKDDDDKV |
Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
Concentration : |
50 μg/mL as determined by BCA |
Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |