Active Recombinant Human LEP protein, Biotinylated

• Cat.No.: LEP-202H
• Product Overview: Biotinylated Recombinant Human LEP protein(NP_000221.1) (Val 22-Cys 167) was expressed in E.coli, with an additional Met.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 22-167 aa
• Conjugation/Label: Biotin
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Immobilized Recombinant Human Leptin Protein, Biotinylated at 2 μg/mL (100 μL/well) can bind Recombinant Human Leptin Receptor / LEPR / CD295 Protein (His & Fc Tag), the EC50 is 45-135 ng/mL.
• Molecular Mass: The recombinant human leptin consists of 147 amino acids and predicts a molecular mass of 16.16 kDa. The apparent molecular mass of rhLEP is approximately 14.5 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: ≥ 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: LEP leptin [ Homo sapiens ]
• Official Symbol: LEP
• Synonyms: LEP; leptin; leptin (murine obesity homolog) , leptin (obesity homolog, mouse) , OB, OBS; obese protein; obesity factor; obese, mouse, homolog of; leptin (murine obesity homolog); leptin (obesity homolog, mouse); OB; OBS; FLJ94114
• Gene ID: 3952
• mRNA Refseq: NM_000230
• Protein Refseq: NP_000221
• UniProt ID: P41159