Recombinant Human LEPR protein, Fc-tagged

• Cat.No.: LEPR-799H
• Product Overview: Recombinant Human LEPR (Phe22-Asp839) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: Leptin receptor (LEPR) is also known as LEP-R, cluster of differentiation 295 (CD295), OB-R and B219, is a single-transmembrane-domain receptor of the gp130 family of cytokine receptors. Leptin receptor exists as homodimer and binds Leptin with high affinity, thus mediates the biological function of the adipocyte-specific hormone Leptin. LEPR is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this protein have been associated with obesity and pituitary dysfunction. Interaction of leptin and leptin receptor is crucial for body weight and bone mass regulation in mammals through hypothalamic effects on satiety and energy expenditure. Meanwhile, research data supports a leptin receptor activation model based on ligand-induced conformational changes.
• Source: HEK293
• Species: Human
• Tag: human IgG1 Fc
• Predicted N Terminal: Phe22
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 121 kDa. The protein migrates as 150-165 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Phe22-Asp839
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: LEPR
• Official Symbol: LEPR
• Synonyms: LEPR; leptin receptor; CD295; OBR; huB219; OB receptor; OB-R; LEP-R; DKFZp686B1731
• Gene ID: 3953
• mRNA Refseq: NM_001003679
• Protein Refseq: NP_001003679
• MIM: 601007
• UniProt ID: P48357