Active Recombinant Human LEPR Protein, hFc-His-tagged

• Cat.No.: LEPR-0357H
• Product Overview: Active Recombinant Human LEPR Protein(NP_002294.2)(20-839 aa) is produced by HEK293 cells expression system, fused with a Fc and His tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: 20-839 aa
• Form: Lyophilized from a 0.22 μm filtered solution of 50mM MES, 100mM NaCl,1mM DTT, pH6.5.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Leptin protein at 2 μg/mL (100 μL/well) can bind Recombinant Human Leptin R/CD295 protein，the EC50 of Leptin R/CD295 is 261.45 ng/mL.
• Molecular Mass: 120.46 kDa
• Endotoxin: < 1 EU/μg of the protein by LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE.
• Storage: Store at -20°C. Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: LEPR leptin receptor [ Homo sapiens ]
• Official Symbol: LEPR
• Synonyms: LEPR; leptin receptor; CD295; OBR; huB219; OB receptor; OB-R; LEP-R; DKFZp686B1731
• Gene ID: 3953
• mRNA Refseq: NM_001003679
• Protein Refseq: NP_001003679
• UniProt ID: P48357