Recombinant Human Leptin Antagonist Triple Mutant

• Cat.No.: LeptintA-15H
• Product Overview: Recombinant Human Leptin Antagonist Triple Mutant is a single non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular weight of 16 kDa, Leptin was mutated, resulting in L39A/D40A/F41A. Leptin Antagonist Triple Mutant Recombinant Human was purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln.
• Physical Appearance: White lyophilized (freeze-dried) powder.
• Purity: Greater than 98.0% as determined by: (a) Gel filtration analysis. (b) Analysis by SDS-PAGE.
• Formulation: The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.0045 mM NaHCO₃.
• Solubility: It is recommended to reconstitute the Recombinant ly Human ophilized Leptin Antagonist Triple Mutant in sterile 0.4% NaHCO₃adjusted to pH 8-9, not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: Leptin triple antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.
• Protein content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.88 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
• Storage: Recombinant Human Lyophilized Leptin Antagonist Triple Mutant although stable at room temperature for several weeks, should be stored desiccated below -18℃. Upon reconstitution at > 0.1 mg/ml and up to 2 mM and filter sterilization Leptin mutant can be stored at 4℃or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.

Gene Information

• Synonyms: OB Protein; Obesity Protein; OBS; Obesity factor; LEP; Lep; leptin; ob; obese; obesity factor; obese, mouse, homolog of; leptin (murine obesity homolog); leptin (obesity homolog, mouse); FLJ94114; Obese protein.