| Species : |
Human |
| Source : |
E.coli |
| Tag : |
Non |
| Description : |
Galectins are a family of animal lectins with an affinity for β-galactosides. There are at least 14 members identified. Galectins share similarities in carbohydrate recognition domain (CRD). Galectins are synthesized as cytosolic proteins. Although localized primarily in the cytoplasm and lacking a classical signal peptide, galectins can also be stimulated to secretion by non-classical pathways or alternatively targeted to the nucleus.Galectins are implicated in modulating cell-cell and cell-matrix interactions. Galectin-3, like all galectins, contains a CRD, while having a distinct chimeric structure with an extra N-terminal tail consisting of 8-13 copies of a 9 aa-repeat consensus sequence that is rich in proline, tyrosine, and glycine. Galectin-3 shares sequence similarity with apoptosis suppressor protein Bcl-2, and due to the Bcl-2-like function,it has been associated with the inhibition of apoptosis and tumor progression. Galectin-3 has also been demonstrated to be involved in heart and skin diseases. |
| Biological Activity : |
The ED50 of rHuGalectin-3 as determined by its ability to agglutinate human red blood cells is 3~10 μg/ml. |
| Purity : |
Greater than 95% as determined by (a) Analysis by RP-HPLC; (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
| Amino-Acid Sequence : |
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Asp-Asn-Phe. |
| Endotoxin : |
Less than 0.1ng/μg (1.0EU/μg) of rHuGalectin-3 as determined by LAL test. |
| Form : |
The protein was lyophilized from a concentrated (1mg/ml) solution in 20mM PBS, pH 7.4. |
| Reconstitution : |
It is recommended to reconstitute the lyophilized rHuGalectin-3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage : |
Lyophilized rHuGalectin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuGalectin-3 should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
