| Description : |
IL-6 activates intracellular signaling through binding a receptor consisting of an ligand-binding protein (IL-6R) and a second protein. IL-6 first binds to IL-6R which subsequently associates with a gp130 dimer. The active signaling complex consists of at minimum IL-6, IL-6R and a dimer of two gp130 proteins that are linked by a disulfide bond. A soluble form of IL-6R is generated by proteolytic cleavage of the membrane-bound precursor and can function as an agonistic molecule that can actively participate in cell-to-cell signaling. The second subunit of the IL-6 complex, gp130, also functions as a component of several additional receptor complexes including leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF) and IL-11. LIF binds to the LIF receptor (LIFR) with low affinity and to a complex of the LIF receptor and gp130 with high affinity while OSM appears to bind to gp130 with low affinity and to a complex of gp130 and the LIF receptor with high affinity. |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
C-His |
| Molecular Mass : |
~87 kDa |
| AA Sequence : |
QKKGAPHDLKCVTNNLQVWNCSWKA PSGTGRGTDYEVCIENRSRSCYQLE KTSIKIPALSHGDYEITINSLHDFG SSTSKFTLNEQNVSLIPDTPEILNL SADFSTSTLYLKWNDRGSVFPHRSN VIWEIKVLRKESMELVKLVTHNTTL NGKDTLHHWSWASDMPLECAIHFVE IRCYIDNLHFSGLEEWSDWSPVKNI SWIPDSQTKVFPQDKVILVGSDITF CCVSQEKVLSALIGHTNCPLIHLDG ENVAIKIRNISVSASSGTNVVFTTE DNIFGTVIFAGYPPDTPQQLNCETH DLKEIICSWNPGRVTALVGPRATSY TLVESFSGKYVRLKRAEAPTNESYQ LLFQMLPNQEIYNFTLNAHNPLGRS QSTILVNITEKVYPHTPTSFKVKDI NSTAVKLSWHLPGNFAKINFLCEIE IKKSNSVQEQRNVTIKGVENSSYLV ALDKLNPYTLYTFRIRCSTETFWKW SKWSNKKQHLTTEASPSKGPDTWRE WSSDGKNLIIYWKPLPINEANGKIL SYNVSCSSDEETQSLSEIPDPQHKA EIRLDKNDYIISVVAKNSVGSSPPS KIASMEIPNDDLKIEQVVGMGKGIL LTWHYDPNMTCDYVIKWCNSSRSEP CLMDWRKVPSNSTETVIESDEFRPG IRYNFFLYGCRNQGYQLLRSMIGYI EELAPIVAPNFTVEDTSADSILVKW EDIPVEELRGFLRGYLFYFGKGERD TSKMRVLESGRSDIKVKNITDISQK TLRIADLQGKTSYHLVLRAYTDGGV GPEKSMYVVTKENS |
| Purity : |
Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE). |
| Notes : |
For research use only, not for use in diagnostic procedure. |
| Storage : |
Store at 4 centigrade short term. Aliquot and store at -20 centigrade long term. Avoid freeze-thaw cycles. |
| Concentration : |
≥0.5 mg/mL |
| Storage Buffer : |
PBS, 4M Urea, pH7.4 |
