Recombinant Human lin-7 homolog B (C. elegans), His-tagged

• Cat.No.: LIN7B-386H
• Product Overview: LIN7B, 1-207aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-207 a.a.
• Description: LIN7B, also known as VELI2, belongs to the Velis family. Velis are a family of small synaptic proteins that interact with other proteins at the post-synaptic density (PSD) of neuronal synapses. Velis contain the PDZ motif involved in recruiting cell adhesion molecules, receptors, and channels. Lin7B is ubiquitously expressed with high expression in brain, liver, and testis. It localized at the synaptic junctions in neurons. Lin7B bind to CASK, a neurexin-binding protein highly concentrated in synapses, and Mint1, a binding partner with a vesicle trafficking protein. Recombinant human LIN7B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol,2mM DTT
• Molecular Mass: 25.3 kDa (230aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAALVEP LGLERDVSRA VELLERLQRS GELPPQKLQA LQRVLQSRFC SAIREVYEQL YDTLDITGSA EIRAHATAKA TVAAFTASEG HAHPRVVELP KTDEGLGFNI MGGKEQNSPI YISRVIPGGV ADRHGGLKRG DQLLSVNGVS VEGEQHEKAV ELLKAAQGSV KLVVRYTPRV LEEMEARFEK MRSARRRQQH QSYSSLESRG
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: LIN7B lin-7 homolog B (C. elegans) [ Homo sapiens ]
• Official Symbol: LIN7B
• Synonyms: LIN7B; lin-7 homolog B (C. elegans); protein lin-7 homolog B; LIN 7B; MALS 2; VELI2; hLin7B; hVeli2; veli-2; vertebrate lin-7 homolog 2; mammalian lin-seven protein 2; MALS2; LIN-7B; MALS-2
• Gene ID: 64130
• mRNA Refseq: NM_022165
• Protein Refseq: NP_071448
• MIM: 612331
• UniProt ID: Q9HAP6
• Chromosome Location: 19q13.3
• Function: protein domain specific binding