Recombinant Human LPA protein, His-tagged

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Recombinant Human LPA protein, His-tagged

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Cat.No. : LPA-7813H
Product Overview : Recombinant Human LPA aa. (Asp4227~Arg4546) fused with N-terminal His tag was produced in E. coli cells.
Description : The protein encoded by this gene is a serine proteinase that inhibits the activity of tissue-type plasminogen activator I. The encoded protein constitutes a substantial portion of lipoprotein(a) and is proteolytically cleaved, resulting in fragments that attach to atherosclerotic lesions and promote thrombogenesis. Elevated plasma levels of this protein are linked to atherosclerosis. Depending on the individual, the encoded protein contains 2-43 copies of kringle-type domains. The allele represented here contains 15 copies of the kringle-type repeats and corresponds to that found in the reference genome sequence.
Source : E. coli
Species : Human
Tag : His
Form : Freeze-dried powder
Molecular Mass : 39kDa as determined by SDS-PAGE reducing conditions.
Protein length : Asp4227~Arg4546
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : >99%
Characteristic : The isoelectric point is 8.4.
Applications : Positive Control; Immunogen; SDS-PAGE; WB.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Concentration : 200μg/mL
Storage buffer : 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300
Reconstitution : Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
SDS-PAGE :

Gene Name : LPA lipoprotein(a) [ Homo sapiens (human) ]
Official Symbol : LPA
Synonyms : LPA; lipoprotein(a); LP; AK38; APOA; apolipoprotein(a); antiangiogenic AK38 protein; apo(a); lipoprotein, Lp(a); lp(a)
Gene ID : 4018
mRNA Refseq : NM_005577.2
Protein Refseq : NP_005568.2

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