Recombinant Human LPL, His-tagged

• Cat.No.: LPL-1014H
• Product Overview: Total 458 AA. MW: 51.61 kDa (calculated). N-Terminal His-tag, 10 extra AA (highlighted).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: LPL is a member of the lipase gene family, which includes pancreatic lipase, hepatic lipase, and endothelial lipase. It is a water soluble enzyme that hydrolyzes triglycerides in lipoproteins, such as those found in chylomicrons and very low-density lipoproteins (VLDL), into two free fatty acids and one monoacylglycerol molecule. It is also involved in promoting the cellular uptake of chylomicron remnants, cholesterol-rich lipoproteins, and free fatty acids. LPL requires Apo-CII as a cofactor.
• Form: Filtered (0. 4 μm) and lyophilized from 0.5 mg/ml in 50mM Acetate beffer, pH=4
• Research topic: Energy metabolism and body weight regulation
• Reconstitution: Add 0.1 M Acetate buffer pH 4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 μg/mL. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: Western blotting
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein; LAL TEST to determine endotoxin level
• Sequences of amino acids: MKHHHHHHAS ADQRRDFIDI ESKFALRTPE DTAEDTCHLI PGVAESVATC HFNHSSKTFM VIHGWTVTGM YESWVPKLVA ALYKREPDSN VIVVDWLSRA QEHYPVSAGY TKLVGQDVAR FINWMEEEFN YPLDNVHLLG YSLGAHAAGI AGSLTNKKVN RITGLDPAGP NFEYAEAPSR LSPDDADFVD VLHTFTRGSP GRSIGIQKPV GHVDIYPNGG TFQPGCNIGE AIRVIAERGL GDVDQLVKCS
• Storage: Store lyophilized protein at -80°C. Lyophilized protein remains stable until the expiry date when stored at -80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.

Gene Information

• Gene Name: LPL lipoprotein lipase [ Homo sapiens ]
• Official Symbol: LPL
• Synonyms: LPL; lipoprotein lipase; LIPD; HDLCQ11; lipoprotein lipase; OTTHUMP00000034806
• Gene ID: 4023
• mRNA Refseq: NM_000237
• Protein Refseq: NP_000228
• MIM: 609708
• UniProt ID: P06858
• Chromosome Location: 8p22
• Pathway: Adipogenesis; Alzheimer"s disease; Chylomicron-mediated lipid transport; Diabetes pathways; Fatty Acid Beta Oxidation; Glycerolipid metabolism; Lipid digestion; Lipoprotein metabolism; Metabolism of lipids and lipoproteins; PPAR signaling pathway; Statin Pathway; Transcriptional Regulation of White Adipocyte Differentiation; Triacylglyceride Synthesis; Vitamin A and carotenoid metabolism
• Function: heparin binding; hydrolase activity; lipoprotein lipase activity; phospholipase activity; protein binding; receptor binding; triglyceride binding; triglyceride lipase activity