Recombinant Human MACC1 protein, His & GST-tagged

• Cat.No.: MACC1-1427H
• Product Overview: Recombinant Human MACC1 aa. (Gln522-Met784 (Accession # Q6ZN28)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: MACC1 is a key regulator of the hepatocyte growth factor (HGF; MIM 142409)-HGF receptor (HGFR, or MET; MIM 164860) pathway, which is involved in cellular growth, epithelial-mesenchymal transition, angiogenesis, cell motility, invasiveness, and metastasis. Expression of MACC1 in colon cancer (MIM 114500) specimens is an independent prognostic indicator for metastasis formation and metastasis-free survival (Stein et al., 2009 [PubMed 19098908]).
• Source: E. coli
• Species: Human
• Tag: His & GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 57.2kDa
• Protein length: Gln522-Met784 (Accession # Q6ZN28)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 8.5

Gene Information

• Gene Name: MACC1 MACC1, MET transcriptional regulator [ Homo sapiens (human) ]
• Official Symbol: MACC1
• Synonyms: MACC1; MACC1, MET transcriptional regulator; 7A5; SH3BP4L; metastasis-associated in colon cancer protein 1; SH3 domain-containing protein 7a5; metastasis associated in colon cancer 1; putative binding protein 7a5
• Gene ID: 346389
• mRNA Refseq: NM_182762.3
• Protein Refseq: NP_877439.3
• UniProt ID: Q6ZN28