Recombinant Human MAD2L1 protein, His-tagged

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Recombinant Human MAD2L1 protein, His-tagged

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Cat.No. : MAD2L1-161H
Product Overview : Recombinant Human MAD2L1 (Met1-Asp205) protein was fused to His-tag at C-terminus and expressed in E.coli.
Description : Mitotic arrest deficient 2-like protein 1 (MAD2L1) is also known as Mitotic spindle assembly checkpoint protein MAD2A (HsMAD2), MAD2. MAD2L1/MAD2 belongs to the MAD2 family. MAD2L1/MAD2 contains one HORMA domain. MAD2L1 is the component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1/MAD2 is required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.
Source : E.coli
Species : Human
Tag : His
Predicted N Terminal : Met1
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 24.3 kDa. The protein migrates as 24 kDa under reducing (R) condition (SDS-PAGE).
Protein length : Met1-Asp205
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of MAD2L1-161H :

Human MAD2L1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Gene Name : MAD2L1
Official Symbol : MAD2L1
Synonyms : MAD2L1; MAD2 mitotic arrest deficient-like 1 (yeast); MAD2 (mitotic arrest deficient, yeast, homolog) like 1; mitotic spindle assembly checkpoint protein MAD2A; HSMAD2; MAD2; MAD2-like protein 1; mitotic arrest deficient 2-like protein 1; mitotic arrest deficient, yeast, homolog-like 1; MAD2 (mitotic arrest deficient, yeast, homolog)-like 1
Gene ID : 4085
mRNA Refseq : NM_002358
Protein Refseq : NP_002349
MIM : 601467
UniProt ID : Q13257

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