Active Recombinant Human MADCAM1 protein, His-Avi-tagged, Biotinylated, Low Endotoxin

• Cat.No.: MADCAM1-201H
• Product Overview: Biotinylated Recombinant Human MADCAM1 protein(Q13477-1)(19-317 aa), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 19-317 aa
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human MADCAM1, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 34.31 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: MADCAM1 mucosal vascular addressin cell adhesion molecule 1 [ Homo sapiens ]
• Official Symbol: MADCAM1
• Synonyms: MADCAM1; mucosal vascular addressin cell adhesion molecule 1; mucosal addressin cell adhesion molecule 1; MACAM1; MAdCAM-1; hMAdCAM-1; mucosal addressin cell adhesion molecule-1
• Gene ID: 8174
• mRNA Refseq: NM_130760
• Protein Refseq: NP_570116
• MIM: 102670
• UniProt ID: Q13477