Recombinant Human MANF Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: MANF-4636H
• Product Overview: MANF MS Standard C13 and N15-labeled recombinant protein (NP_006001) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 21.6 kDa
• AA Sequence: MRRMRRMWATQGLAVALALSVLPGS RALRPGDCEVCISYLGRFYQDLKDR DVTFSPATIENELIKFCREARGKEN RLCYYIGATDDAATKIINEVSKPLA HHIPVEKICEKLKKKDSQICELKYD KQIDLSTVDLKKLRVKELKKILDDW GETCKGCAEKSDYIRKINELMPKYA PKAASARTDLTRTRPLEQKLISEED LAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: MANF mesencephalic astrocyte-derived neurotrophic factor [ Homo sapiens (human) ]
• Official Symbol: MANF
• Synonyms: MANF; mesencephalic astrocyte-derived neurotrophic factor; arginine rich, mutated in early stage tumors, ARMET; ARP; arginine-rich, mutated in early stage tumors; ARMET; MGC142148; MGC142150
• Gene ID: 7873
• mRNA Refseq: NM_006010
• Protein Refseq: NP_006001
• MIM: 601916
• UniProt ID: P55145