Recombinant Human MAP2K3 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: MAP2K3-2270H
• Product Overview: MAP2K3 MS Standard C13 and N15-labeled recombinant protein (NP_002747) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 36.6 kDa
• AA Sequence: MSKPPAPNPTPPRNLDSRTFITIGD RNFEVEADDLVTISELGRGAYGVVE KVRHAQSGTIMAVKRIRATVNSQEQ KRLLMDLDINMRTVDCFYTVTFYGA LFREGDVWICMELMDTSLDKFYRKV LDKNMTIPEDILGEIAVSIVRALEH LHSKLSVIHRDVKPSNVLINKEGHV KMCDFGISGYLVDSVAKTMDAGCKP YMAPERINPELNQKGYNVKSDVWSL GITMIEMAILRFPYESWGTPFQQLK QVVEEPSPQLPADRFSPEFVDFTAQ CLRKNPAERMSYLELMEHPFFTLHK TKKTDIAAFVKEILGEDSTRTRPLE QKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: MAP2K3 mitogen-activated protein kinase kinase 3 [ Homo sapiens (human) ]
• Official Symbol: MAP2K3
• Synonyms: MAP2K3; mitogen-activated protein kinase kinase 3; PRKMK3; dual specificity mitogen-activated protein kinase kinase 3; dual specificity mitogen activated protein kinase kinase 3; MAP kinase kinase 3; MAPK/ERK kinase 3; MAPKK3; MEK3; MKK3; MEK 3; MAPKK 3; SAPK kinase 2; stress-activated protein kinase kinase 2; SAPKK2; SAPKK-2
• Gene ID: 5606
• mRNA Refseq: NM_002756
• Protein Refseq: NP_002747
• MIM: 602315
• UniProt ID: P46734