Recombinant Human MAP4K5 protein, His & T7-tagged

• Cat.No.: MAP4K5-1536H
• Product Overview: Recombinant Human MAP4K5 aa. (Thr613~His842 (Accession # Q9Y4K4)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Thr613~His842
• Description: This gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 29.8kDa.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method).
• Purity: >95%
• Characteristic: The isoelectric point is 6.9.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: MAP4K5 mitogen-activated protein kinase kinase kinase kinase 5 [ Homo sapiens (human) ]
• Official Symbol: MAP4K5
• Synonyms: KHS; GCKR; KHS1; MAPKKKK5; MAPK/ERK kinase kinase kinase 5; MEK kinase kinase 5; MEKKK 5; germinal center kinase-related; kinase homologous to SPS1/STE20; mitogen-activated protein kinase kinase kinase kinase 5
• Gene ID: 11183
• mRNA Refseq: NM_006575.4
• Protein Refseq: NP_006566.2
• UniProt ID: Q9Y4K4